DNA

Part:BBa_K4051022:Design

Designed by: Clodagh Healy   Group: iGEM21_MTU-CORK   (2021-10-11)


PCRA GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1318
    Illegal BamHI site found at 177
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2816
    Illegal AgeI site found at 1127
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

codon optimised for E.coli


Source

Generic Histag used

Generic Secretion Signal from Gaussian luciferase

References